在一根3m长的色谱柱上分离一个试样的结果如下:死时间为1min,组分1的保留时间为14min,组分2的保留时间为17min,峰宽为1min。(1) 用组分2计算色谱柱的理论塔板数n 及塔板高度H mm , (2)求调整保留时间tr1’ min 及tr2’ min ;(3)求相对保留值r2,1 和分离度R 。
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