【单选题】Cloning vectors with the transcription and translation signals needed for the regulated expression of a cloned gene are called expression vectors. Which of the following statements about expression ve...
A.
The rate of expression of the cloned gene is controlled by replacing the gene’s normal promoter and regulatory sequences with more efficient and convenient versions supplied by the vector.
B.
Generally, a well-characterized promoter and its regulatory elements are positioned near several unique restriction sites for cloning, so that genes inserted at the restriction sites will be expressed from the regulated promoter elements.
C.
In some cases, cloned genes are so efficiently expressed that their protein product represents 10% or more of the cellular protein. At these concentrations, some foreign proteins can kill the host cell (usually E. coli ), so expression of the cloned gene must be limited to 24 hours before the planned harvesting of the cells.
D.
Some of these vectors incorporate other features, such as a bacterial ribosome-binding site, to enhance translation of the mRNA derived from the gene or a transcription termination sequence.