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Lysozyme is an important component of the human innate immune system. Lysozyme is an enzyme that cleaves glycosidic linkages in bacterial cell walls. The pH optimum of the purified enzyme is 5.2. There are two acidic residues at the active site of lysozyme (E35 and D52) that are required for enzyme activity. The pKa of E35 is 5.9, whereas the pKa of D52 is 4.5. What are the primary ionization states of these two residues at the pH optimum of the enzyme?
A.
E35 is protonated; D52 is ionized.
B.
E35 is protonated; D52 is protonated.
C.
E35 is ionized; D52 is protonated.
D.
E25 is ionized; D52 is ionized.
E.
This cannot be determined from the information provided.