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【单选题】
某企业运用原有技术优势,开发了一种固定资产投资极大的新产品,投产后很畅销。几家竞争对手看到该产品有巨大潜力,也纷纷跃跃欲试。这时,有人从资料中发现该产品完全可以通过其他途径加以合成,而投资仅为原来五分之一。该企业顿时陷入手忙脚乱的境地。通过计划过程分析,该企业最有可能在以下哪个环节上出了“毛病” ? ( )A.
明确计划的前提条件
B.
估量机会，确立目标
C.
拟定派生计划，并通过预算使计划数字化
D.
提出备选方案，经过比较分析，确定最佳方案
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反馈参考答案：
举一反三
【单选题】I wanted to have him ______ the job, but she had it _____ already.
A.
do, done
B.
does, doing
C.
to do, doing
D.
to do, done
【简答题】重组载体疫苗(recombinant vector vaccine)(名词解释)
【简答题】微机控制点火系中最佳点火提前角由 、 、 组成。
【单选题】I wanted to have him _____ the job, but she had it _____ instead.
A.
do/done
B.
does/doing
C.
to do/doing
【多选题】How is recombinant DNA cloned in bacteria?
A.
To clone a particular fragment of DNA, it is first inserted into a plasmid vector. To insert a piece of DNA into a plasmid vector, the purified plasmid DNA is opened up by a restriction enzyme that cleaves it at a single site, and the DNA fragment to be cloned is then spliced into that site using DNA ligase. This recombinant DNA molecule is now ready to be introduced into a bacterium, where it will be copied and amplified.
B.
The resulting recombinant plasmid DNA is then introduced into a bacterium by transformation. These bacteria are then suspended in a nutrient-rich broth and allowed to proliferate.
C.
In 24 hours, the engineered cells will produce hundreds of millions of copies of the plasmid, along with the DNA fragment it contains. The bacteria can then be split open (lysed) and the plasmid DNA purified from the rest of the cell contents, including the large bacterial chromosome.
D.
The DNA fragment can be readily recovered by cutting it out of the plasmid DNA with the same restriction enzyme that was used to insert it, and then separating it from the plasmid DNA by gel electrophoresis. Together, these steps allow the amplification and purification of any segment of DNA from the genome of any organism.
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