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【简答题】
用pH梯度萃取法分离生物碱，具体操作方法有两种：一是将总生物碱溶于酸水，逐步加______使pH由______到______，每调一次pH，用三氯甲烷等亲脂性有机溶剂萃取一次，将生物碱按碱性由______到______的顺序分离。另一种方法是将总碱溶于三氯甲烷中，用pH由______到______的水溶液依次萃取，将生物碱按碱性由______到______的顺序进行分离。
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