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研究者发现,舌头上的某些味觉细胞和小肠上皮细胞表面均存在蛋白C,该蛋白能和脂肪结合。为研究蛋白C的功能,进行了系列实验。 (1) 蛋白C是一种膜蛋白,它在细胞内的 上合成,然后在 和 中加工。 (2) 实验一:让舌头接触脂肪,结果发现正常小肠出现脂肪消化液,而去除蛋白C基因的分泌的脂肪消化液明显减少。由此推测,脂肪通过与味觉细胞表面的蛋白C结合,刺激了脂肪味觉 ,产生兴奋,传到相关中枢,再通过 刺激消化腺分泌。 (3) 实验二:分别培养实脸一中两种的小肠上皮细胞,向培养液中加入脂肪分解物。与正常细胞相比,进入去除蛋白C基因的细胞的脂肪分解物减少,表明小肠上皮细胞表面蛋白C的功能是 。 (4) 为了证实其他哺乳动物的蛋白C也有相似作用,可行的做法是从该种动物的基因文库中 蛋白C基因序列,然后以 的为受体,导入该基因序列,检测发育出的相关指标的恢复程度。
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